| ORIGINAL ARTICLE |
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| Year : 2022 | Volume
: 16
| Issue : 4 | Page : 306-311 |
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Microbial study of Bhallatakarishta formulate by two different liquid media
Dharmishtha Bopaliya1, Dipali Parekh2, Meera Cholera3, Biswajyoti Patgiri2
1 Department of Rasa Shastra and Bhaishajya Kalpana, Indian Institute of Ayurved Research and Hospital, Rajkot, Gujarat, India
2 Department of Rasa Shastra and Bhaishajya Kalpana, ITRA, Jamnagar, Gujarat, India
3 Department of Microbiology, ITRA, Jamnagar, Gujarat, India
Correspondence Address:
Dharmishtha Bopaliya
Department of Rasa Shastra and Bhaishajya Kalpana, Indian Institute of Ayurved Research and Hospital, Rajkot, Gujarat
India
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/joa.joa_255_21
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Introduction: Asava and Arishta (fermented formulations) are unique dosage forms of Ayurveda due to their indefinite shelf-life. The self-generated alcohol of these preparations is potentiating both the level pharmaceutically and therapeutically. Aim: The aim of the study is to carry out the stability of Bhallatakarishta (BA) prepared by two different liquid media with respect to its stability against microbial contamination. Methods: Both these BA samples were prepared and studied to check for microbial contamination at regular intervals. Results: Each time, the sample was subjected in microbial study from the day of preparation to the date of the last microbial study. No contamination was found in a microbial study in water media. In the one batch of Kanji (fermented sour gruel) media out of two batches, microbes were found after the 5th month date of preparations. Discussion: A current study was conducted to observe the stability of BA regarding microbial contamination for sample preparation and storage under different climatic conditions and temperatures. Thus, the basic microbial profile was studied at regular intervals for the consumption of the prepared drug. At the end of the study, it was found that the samples were not shown for the presence of any microorganisms in most of the collections. Conclusion: During the study period, no microorganisms were isolated from aerobic culture, and no fungal pathogens were identified from fungal culture in any batches of water media. Thus, these data support the long shelf-life of Asava–Arishta formulation.
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